TY - BOOK AU - Lympany,Penny AU - Jones,Meinir G. TI - Allergy: methods and protocols T2 - Methods in molecular biology, SN - 9781493995912 AV - RC584 U1 - 616.97 23 PY - 2019/// CY - New York, NY PB - Humana Press KW - Allergy KW - Laboratory manuals KW - Electronic books N1 - Includes index; Academic; Preface…Table of Contents…Contributing Authors… 1. Understanding of the Molecular Mechanisms of AllergyMeinir Jones 2. T-Cell: Primary Culture from Peripheral BloodMonika Raulf 3. Measurement of Allergen-Specific Inhibitory Antibody ActivityMohamed H. Shamji and James N. Francis 4. Immunoelectrophoresis for the Characterization of Allergen ExtractsGitte Nordskov Hansen and Jørgen Nedergaard Larsen 5. Standardization of Allergen ExtractsJørgen Nedergaard Larsen and Sten Dreborg 6. Quantifying Human Innate Cytokine and Chemokine Responses Ex Vivo via Pattern Recognition Receptor StimulationNatascha Fitch, Stephanie J. Marshall, William P. Stefura, Rishma Chooniedass, Allan B. Becker, and Kent T. HayGlass 7. Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive ELISA and Meso Scale Electrochemiluminescence AssaysWilliam P. Stefura, Caroline Graham, Larisa Lotoski, and Kent T. HayGlass 8; Development of Mould Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen ExposureEva Zahradnik and Monika Raulf 9. Monocyte-Derived Dendritic Cells as Antigen Presenting Cells in T-Cell Proliferation and Cytokine ProductionSun-sang J. Sung 10. The Use of Dual Cell Tracker Dye Staining for the Identification and Characterization of Peanut Specific T-Cell SubsetsCecilia Dunsterville, Alick C. Stephens, Gideon Lack, and Victor Turcanu 11. The Use of pMHCII Tetramer Technology for the Identification and Characterization of CD4+ T CellsErik Wambre 12. Isolation and Characterization of Lymphocytes from Human Mucosal BiopsiesNadine E.G. Upton, Thomas S. Hayday, and Louisa K. James 13. Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen ExposureDaniel Álvarez-Simón and Susana Gómez-Ollés 14; Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel InhibitorsJihui Zhang and Clive Robinson 15. Measurement of Specific IgG Anti-Fel d 1 AntibodiesMeinir Jones 16. Measurement of Specific IgG4 Anti-Mouse Urine AntibodiesJennifer Canizales 17. Preparation of Samples for a Mass Spectrometry Based Method to Identify Allergenic ProteinsMary Brian; Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK) UR - https://online.fliphtml5.com/lluzx/mqzr/ ER -